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Image Search Results


Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

doi: 10.3389/fcell.2026.1929409

Figure Lengend Snippet: Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.

Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

Techniques: Expressing, Western Blot, Analysis, Derivative Assay

Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

doi: 10.3389/fcell.2026.1929409

Figure Lengend Snippet: Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.

Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

Techniques: Expressing, Analysis, Quantitative RT-PCR, Derivative Assay, Western Blot